Regeneration and Recycling of Supports for Biological Macromolecules Purification

Great evolution and improvement in biological molecules purification have been achieved in the last 20 years, giving advantages in both product quality and yield, and speed of purification methods. Purity levels, once poorly suitable for large scale production, as well as requiring long and very expensive procedures, are today achievable using simple procedures. For example, till few decades ago, the only way to obtain ultrapure nucleic acids was the ultracentrifugation on cesium chloride gradient; today, the same -if not higherpurity is easily achieved using solid-phase anion-exchange separations. The purification of recombinant proteins has also been dramatically improved by using more precise affinity techniques.